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The gel-like to liquid crystalline phase transition is clearly reflected by the relatively sudden inc.r.ea.sc in wavenumber of the CH2 stretching band around 2850 cm in the IR absorption spectrum or by the decreuse in wavenumber of the C=O stretching band contour around 1735 cm I. The increasing wavenumber of the CH2 stretching band reflects a decrease in the chain order. Besides, the bandwidth suddenly changes as a result of the mobility change when the acyl chains undergo phase transition. The Raman spectra show other consistent changes, in particular the increasing intensity ratios 1(2850)/1(2880) and 1(2932)/I(2880) (Snyder et al., 1980; Litman et al., 1991). The C=O stretching band in the 1R absorption spectrum is equally useful. Phase transition shifts this band from 1738 cm towards lower wavenumbers; it is found around 1733 cm as phospholipid vesicles undergo a gel to liquid crystalline phase transition Tm. Mantsch interpreted the shift towards lower wavenumbers of the ovcrall band contour peak as resulting from the intensity increase of'the component at 1727 cm upon a change in hydration (by hydrogen bonding) during the transition (Mantsch and Mc Elhaney, 1991). Finally, it should be mentioned that the temperature profile may indicate the possible existence of heterogeneities or microdomains. Raman spectra have shown such phase separations in bilayers prepared with DHPC/DPPC mixtures and in lipids containing highly unsaturated sn2-acyl and completely saturated snl-acyl, such as POPC, PAPC, and PDPC (Litman et al., 1991). WI 0 room 1
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