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Okay, get into writing the parser module. Create a new file in your project directory named minifeedparser.py and start off with the code shown in Listing 2-10.
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When invoking a Troi plug-in that you haven t yet registered (that is, paid for and activated by entering the registration code), you ll see an alert reminding you to register. You can dismiss this dialog by clicking in it; then the plug-in will run.
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These chapter markers give viewers a rudimentary way of navigating quickly through the movie on your DVD. But if you want to create a proper scene-selection menu tied to chapter markers that appear where you want them to, you need to do a bit more work, as described next.
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The structure of the frame relay link management messages looks very much like the structure of the frame relay signaling messages described in 5. This is no accident, of course, because to the ITU-T and ANSI, link management messages are only other kinds of signaling (i.e., nonuser information-carrying) frames. The section will detail the structure of the ANSI and ITU-T Status Enquiry and Status messages. Some mention will be made of the LMI message formats, but only in passing. The overall structure of the ITU-T Q.933 Annex A and ANSI T1.617 Annex D Status Enquiry message is shown in Figure 7.3. Note the similarities between the two. Consider the ITU-T Annex A format first. The DLCI is 0 and the Control field value of 3 in hexadecimal (03h) indicates that this is an unnumbered information (UI) frame (regular signaling messages are sent as Information [I] frames that have a send and receive sequence field in the Control field). The Protocol Discriminator field which follows is still present and used to identify the exact signaling protocol used. As in all frame relay signaling messages based on Q.931, this field is set to 00001000, which is nothing more than the number 8 in hexadecimal (08h). The next octet in the Status Enquiry message header forms the Call Reference field. The first four bits of this field are always 0000 and the next four bits give the exact length of the call reference value itself. For link management messages, the Call Reference value is always exactly zero in all 8-bit positions. The all-zero Call Reference not only means that the length of the Call Reference field is just one octet and this is it; but it also serves as a Dummy (the term is used consistently in the standards) Call Reference value. The Dummy Call Reference makes sense in link management messages because the Call Reference value is used to track demand connections internally on the frame relay network. However, there is no connection to track in a link management message exchange of Status Enquiry and Status messages, so the Dummy Call Reference value keeps the field intact but lets the network effectively ignore it.
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Although the History panel enables you to replay any series of selected steps at the click of a button, you have to click that button every time you want to replay the steps. I developed a custom extension called Repeat History with which you can repeat selected steps any number of times. You ll find Repeat History in the Additional Extensions folder on the CD-ROM.
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differentiation provides an opportunity to explore the molecular processes that control cell differentiation in the early human embryo. Despite their resemblance to cells within the early-stage embryo, it is always important to remember that EC cells are derived from tumors. As a consequence, EC cells are most often aneuploid and possess an abnormal karyotype. Furthermore, there is a strong selective pressure for the growth of EC cells in tumors that have reduced potential for differentiation since cell differentiation itself leads to cells with restricted proliferative ability. As a consequence, many EC cell lines possess a reduced capacity for cell differentiation compared to their normal embryonic counterparts. In some ways, however, this can be an advantage depending on the context in which the experimental model is used. For example, there are many cases in which cultured murine and human EC cells are used to study the process of neural differentiation [Andrews, 1984; Jones-Villeneuve et al., 1982; Macpherson et al., 1995; McBurney et al., 1988; Przyborski et al., 2000; Stewart et al., 2003; see also 3]. The more restricted developmental potential of certain EC cell lines can offer an alternative to primary cells, where consistency and quantity of material are variable, and embryonic stem cells, which are technically more challenging to grow and have the tendency to spontaneously differentiate and form progeny representative of all three germ layers. Research using human EC cells as models of early embryogenesis remains a useful, viable alternative and serves as a simple and robust experimental system to investigate, for example, cell fate determination in the embryonic ectoderm [see for review Przyborski et al., 2004]. 6.1.2. Culture of Human Embryonal Carcinoma Cell Lines In general, it is possible to isolate and establish EC cell lines from teratocarcinomas whatever their origin providing there is a resident population of proliferating stem cells. Another generalization is the morphology of such cells, in that all murine and human EC lineages derived so far share a characteristic structure of a sparse ring of cytoplasm surrounding a rounded nucleus with one or two prominent nucleoli. Moreover, such morphology closely resembles that of embryonic stem cells. EC cells generally grow as tightly packed colonies, with cells adhering strongly to one another, and often the boundaries between cells are indistinct. Many of the widely used EC cell lines have adapted to grow independent of feeder cells. Thus it is technically less challenging to grow EC cells routinely, and this allows the generation of large quantities of homogeneous cellular material. In general, the requirements for culture of human EC cells are modest compared to human embryonic stem cells, in that specialized (and often expensive) media supplements and feeder cells are not needed. Dulbecco s modi ed Eagle s medium (high-glucose formulation supplemented with 2 mM l-glutamine) is most commonly used as a base medium to grow EC cells, although other investigators have preferred the -modi cation of Eagle s minimal essential medium ( -MEM). The medium is usually supplemented by 10% heat-inactivated fetal bovine serum (FBS). It is recommended that different batches of FBS from alternative suppliers are screened to select a batch that is optimal for growth and differentiation by examining several for EC cell growth and ability to differentiate before selection of the batch. We look at the expression of cell surface stem cell markers (SSEA-3, TRA1-60) and differentiation markers (VINIS, A2B5, after 7-day retinoic acid exposure) by ow cytometry (see Protocol 6.5). Standard tissue culture plastic asks and multiwelled plates from any of the major suppliers of consumables are generally satisfactory for
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[chieh@penguin home]$ dir total 132 drwxr-xr-x 2 4096 Mar 11 00:28 ./ drwxr-xr-x 20 4096 Mar 11 00:28 ../ -rw-r--r-1 122687 Mar 10 23:58 photopc-3.05.tar.gz [chieh@penguin home]$ tar xvzf photopc-3.05.tar.gz
FIGURE 8-2: The lens is flush with the plastic tube when fully extended.
6. Click the OK button to dismiss the Conditional Formatting dialog. 7. With the asterisk text object still selected, choose Format Text Color and select a light gray to match the color of the tab panel behind the asterisk.
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1. Select the path by clicking it with the Direct Selection tool. You can also select multiple paths and then modify them one at a time. 2. Select the Pen tool, the Add Anchor Point tool, or the Delete Anchor Point tool. You can use any of these tools to add and delete anchor points. If the Type tool is not selected, you can select the Pen tool by pressing P, the Add Anchor Point tool by pressing =, and the Delete Anchor Point tool by pressing (hyphen) on the main keyboard or on the numeric keypad. 3. Move the pointer over the anchor point that you want to delete and then click. If the Add Anchor Point tool is selected, you must hold down the or Ctrl key to delete an anchor point. Figure 27.15 shows a before-and-after example of a path from which an anchor point has been deleted.
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I cover charting in s 19 and 20.
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