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The isolation between the modes helps in maintaining a high isolation between channels, thus relaxing the requirements on frequency filters [Guy and Davies 1983]. Connecting the phase mode ports of the Butler matrix to a second network, as shown in Figure 9.8 [Sheleg 1968], makes it possible to create any (physical) beam pattern using phase-mode synthesis. The variable phase shifters can be used for scanning the beam since from one mode to the next will shift the excitation adding a phase shift with increments and also the radiation pattern by the same amount in angle. This fact, noted by Davies [1965], follows directly from the phase mode discussion in 2. Let us revisit Equation (2.22) describing the far field radiation expressed in phase modes with amplitudes Am: E( ) =
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94. N. C. Billingham, in Developments in Polymerisation-l, R. N. Haward, ed., Applied Science Publishers, London, 1979, 4, p. 147. 95. V. Dragutan, A. T. Balaban, and M. Dimonie, Olefin Metathesis and Ring-Opening Polymerization of Cyclo-Olefins, Editura Academiei, Bucuresti, and Wiley, Chichester, 1985, 4, p. 155. 96. R. Grubbs and B. M. Novak, in Encyclopedia of Polymer Science and Engineering, 2nd ed., Supplement Volume, J. 1. Kroschwitz, ed., Wiley-Interscience, New York, 1989, p.420. 97. R. H. Grubbs and W. Tumas, Science 243,907 (1989). 98. A. J. Amass, in Comprehensive Polymer Science, G. Allen, J. C. Bevington, eds., Vol. 4: Chain Polymerization II, G. C. Eastmond, A. Ledwith, S. Russo, and P. Sigwalt, eds., Pergamon Press, Oxford, 1989, 6, p. 109. 99. H. R. Kricheldorf, in Handbook of Polymer Synthesis, H. R. Kricheldorf, ed., Marcel Dekker, New York, 1992, 7, p. 433. 100. R. R. Schrock, Acc. Chem. Res. 23, 158 (1990). 101. J. Feldman and R. R. Schrock, Prog. Inorg. Chem. 39, 1 (1991). 102. B. M. Novak, W. Risse, and R. H. Grubbs, Adv. Polym. Sci. 102,47 (1992). 103. G. C. Bazan, R. R. Schrock, H.-N. Cho, and V. C. Gibson, Macromolecules 24, 4495 (1991). 104. R. R. Schrock, S. A. Krouse, K. Knoll, J. Feldman, J. S. Murdzek, and D. C. Yang, J. Mol. Catal. 46, 243 (1988). 105. J. Kress, J. A. Osborn, R. M. E. Greene, K. J. Ivin, and J. J. Rooney, J. Chem. Soc., Chem. Commun. 874 (1985). 106. R. R. Schrock, J. Feldman, L. F. Cannizzo, and R. H. Grubbs, Macromolecules 20,1169 (1987). 107. L. L. Blosch, K. Abboud, and J. M. Boncella, J. Am. Chem. Soc. 113, 7066 (1991). 108. J.-L. Couturier, C. Paillet, M. Leconte, J.-M. Basset, and K. Weiss, Angew. Chem., Int. Ed. Engl. 31, 628 (1992). 109. P.A. van der Schaaf, W. J. J. Smeets, A. L. Spek, and G. van Koten, J. Chem. Soc., Chem. Commun. 717 (1992). 110. L. R. Gilliom and R. H. Grubbs, J. Am. Chem. Soc. 108, 733 (1986). 111. K. C. Wallace and R. R. Schrock, Macromolecules 20, 448 (1987). 112. K. C. Wallace, A. H. Liu, J. C. Dewan, and R. R. Schrock, J. Am. Chem. Soc. 110,4964 (1988). 113. R. Toreki, R. R. Schrock, and M. G. Vale, J. Am. Chem. Soc. 113,3610 (1991). 114. N. A. Petasis and D.-K. Fu, J. Am. Chem. Soc. 115, 7208 (1993). 115. T. J. Katz, S. J. Lee, and N. Acton, Tetrahedron Lett. 4247 (1976). 116. J. G. Hamilton, K. J. Ivin, and J. J. Rooney, J. Mol. Catal. 28, 255 (1985). 117. H. H. Thoi, K. J. Ivin, and J. J. Rooney, J. Mol. Catal. 15,245 (1982). 118. J. G. Hamilton, K. J. Ivin, G. M. McCann, and J. J. Rooney, J. Chem. Soc., Chem. Commun. 1379 (1984). 119. W. A. Herrmann, W. Wagner, U. N. Flessner, U. Volkhardt, and H. Komber, Angew. Chem., Int. Ed. Engl. 30, 1636 (1991). 120. M. McCann and D. McDonnell, J. Chem. Soc., Chem. Commun. 1718 (1993).
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Following Check In/Check Out Procedures ..............................................................................944 Check In/Check Out overview ......................................................................................944 Enabling Check In/Check Out ......................................................................................946 Checking files in and out ..............................................................................................947 Keeping Track with Design Notes ............................................................................................949 Setting Up for Design Notes ..........................................................................................950 Setting the status with Design Notes ..............................................................................951 Creating custom Design Notes ......................................................................................952 Viewing Design Notes ....................................................................................................953 Browsing File View Columns ....................................................................................................954 Generating Reports ..................................................................................................................956 Outputting HTML Reports ............................................................................................958 Using Workflow reports ................................................................................................959 Administering Adobe Contribute Sites ....................................................................................960 Setting Up Contribute Compatibility ..............................................................................962 Entering sitewide administrator settings ........................................................................963 Establishing Contribute roles ........................................................................................965 Connecting users ..........................................................................................................975 Rolling back a Contribute page in Dreamweaver ............................................................978 Integrating Dreamweaver with Visual SourceSafe ....................................................................980 Communicating with WebDAV ................................................................................................981 Summary ..................................................................................................................................983
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overview of the methods used in metagenomic Environmental sample studies. If you compare the nucleic acid-based community analysis already described in Figure 6.7 Metagenomic library construction with Figures 6.9 and 6.10, the major contrasts are Transform into a clear: Extract host bacterium Clone 1 In metagenomics there is an expansion of interDNA (e.g., E. coli) est from small subunit rRNA to all genes. 2 In metagenomics there is no PCR step that can Metagenomic library distort the relative abundances of DNA fragments. 3 The size of the DNA fragment sorted into cloning Metagenomic vectors can be small [~3 kilobases (kb)] in the shotanalysis gun cloning approach or very large (~40 100 kb) Random Screen for in the bacterial arti cial chromosome (BAC) and Screen for particular sequencing expression of sequences by PCR cosmid/fosmid cloning approaches. particular phenotypes or hybridization 4 The large (BAC or cosmid/fosmid) DNA fragments are subcloned, sequenced, and assembled into a Figure 6.9 Overview of metagenomics: single genomic fragment representing a portion of construction of a DNA library from mixed the genome from a single microorganism. Thus, microbial populations in an environmental associations between genes (especially 16S rRNA sample and three approaches for analyzing and functional genes) can be determined; these the sequences to reach conclusions about gene content. Both taxonomic (i.e., small gene associations re ect those in a single unculsubunit rRNA) and functional gene tured host residing in the sampled community. sequences are analyzed. The function of 5 The short (shotgun) DNA fragments are derived some genes can be con rmed by cloning from genetically heterogeneous populations. and expressing them (as proteins) in Even with hundreds or thousands of 3 kb pieces bacterial hosts such as E. coli. PCR, of cloned DNA (that contribute to thorough polymerase chain reaction. (From clone coverage ), the sequences from shotgun Riesenfeld, C.S., D.D. Schloss, and cloning may not be able to be assembled because J. Handelsman. 2004. Metagenomics: the 3 kb sequencing lengths from complex genomic analysis of microbial communities. communities may not share suf cient sequence Annu. Rev. Genet. 38:525 552. Reprinted similarity to be aligned to create coherent con- with permission from Annual Review of Genetics, Vol. 38. Copyright 2004 by tiguous genomic fragments (contigs). Annual Reviews, www.annualreviews.org.) As shown in Figure 6.9, after the environmental DNA has been incorporated into a cloning vector and a bacterial host, there are three distinctive strategies for continuing the metagenomic analysis (Riesenfeld et al., 2004): 1 The random (shotgun) sequencing approach uses plasmids as vectors, and thousands of ~3 kb inserts represented in the clone library are sequenced. Such high throughout sequencing projects (e.g., rst three entries in Table 6.7) utilize instrumentation and facilities developed to sequence the human genome. The monetary expense of sequencing does not in uence project implementation the investigators do it all . It follows then, that the shotgun approach to metagenomics generates immense DNA-sequencing data sets. Consistent with this, note that the clones obtained in entries 1 3 of Table 6.7 were not screened for
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