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Reagents and Materials Sterile or aseptically prepared Counted cell suspension Growth medium (See Section 4) Matrigel Scaffold disks (as in Protocol 6.1) Petri dishes, 9 cm Kimwipes Protocol (a) Thaw Matrigel overnight in a 4 C refrigerator. (b) Prewet the scaffolds with culture medium and place into a 37 C, 5% CO2 incubator for 2 h before inoculation. (c) Blot-dry the scaffolds with sterile Kimwipes, and transfer them to empty Petri dishes (4 5 scaffolds/dish.)
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aVeCh(L:~=l AiXB;) = L (~B~ 0
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V= Est[var(t1y )] Est[cov(t1y t2y )] Est[var(t2y )] .. . Est[cov(t1y tpy )] . . . , Est[cov(tp 1y tpy )] Est[var(tpy )]
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Figure 8.8. Identi cation results for Example 3.
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Figure 7.5 GaAs HBT emitter base (E B) back-to-back ESD diode string transmission line pulse (TLP) I V characteristic
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Table 5.7 B M algorithm table, Example 5.10 1 0 1 2 3 4
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