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printf( Error - Invalid WAV file!\n ); exit(0); } //if // Read data in overt2... read_cnt = fread( charr, sizeof(char), 4, overt2Stream ); if(read_cnt < 4) { printf( Error - Invalid WAV file! ); exit(0); } //if charr[4] = 0; if(!strcmp(charr, data ) == 0) { printf( Error - Invalid WAV file!\n ); exit(0); } //if // Read data size in overt2... read_cnt = fread( charr, sizeof(char), 4, overt2Stream ); if(read_cnt < 4) { printf( Error - Invalid WAV file!\n ); exit(0); } //if
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Personality, 234 251 clinical inference and judgment, 251 254 communicating assessment results, 254 258 case study, 255 257 integrated psychological assessment report, 257 258 outline of typical psychological report, 254 defining personality and psychological functioning, 234 235 disorders, 191, 470 471 enduring nature of, 236 genetic component of traits, 150 measuring, 236 254 objective testing: Edwards Personal Preference Schedule (EPPS), 242 Eysenck Personality Questionnaire, 242 MCMI, 239 241 MMPI, 237 239 (see also Minnesota Multiphasic Personality Inventory (MMPI-2)) NEO-Personality InventoryRevised (NEO-PI-R), 241 242 16PF, 241, 242 overview, 258 260 projective testing, 236, 242 248 projective drawings, 246 247 Rorschach, 242 245 sentence completion techniques, 248 Thematic Apperception Test (TAT), 245 246 questions/controversies concerning personality and psychological testing, 248 251 employment decisions and psychological testing, 251
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a representative marker for cell matrix production. RGD coupling, through the enhancement of initial cell density, induced changes in collagen type I production over time as determined by mRNA transcript (monitored via semiquantitative RTPCR) levels at day 7 and protein concentration at day 14. The signi cant increases observed in collagen type I mRNA levels from BMSCs and ACLFs grown on RGDmodi ed matrices are likely the result of an increase in cell density; enhanced cellcell interactions likely induced a switch from cell proliferation to matrix production (differentiation). The increase in collagen type I transcript levels from BMSCs was vefold greater than the increase observed from ACLFs (i.e., 180% compared with 30%). ELISA against collagen type I at day 14 con rmed the trends observed in transcription levels at day 7. By 14 days of culture, a 410% increase in BMSC collagen type I production on RGD-modi ed compared with nonmodi ed matrices was observed, supporting the notion of a switch from a proliferative nondifferentiated state at low cell density (e.g., 7 days) to a matrix-producing state at high cell densities (14 days). Furthermore, although the effects on collagen type I matrix production may simply be the result of an increase in cell density, the introduction of additional cell binding sites through RGD modi cation may also induce subsequent intracellular signaling events to promote cell matrix production and differentiation [Grzesiak et al., 1997]. For tissue engineering, BMSCs should attach rmly on silk matrices, and, appropriately, most BMSCs remained on the RGD-modi ed silk lm after trypsinization for 15 min, whereas most of the cells seeded on tissue culture plastic and EDC/NHS-treated and nonmodi ed silk lms detached. These data are consistent with several studies that indicated strong adhesion of cells to RGD peptides on surfaces [Massia and Hubbell, 1990; Neff et al., 1999]. The present study has begun to address these issues by demonstrating a role for RGD-modi ed silk bers in accelerating the attachment, coverage, and differentiation of BMSCs. These studies extend our prior work [Altman et al., 2002a, b] and suggest a path forward for continued optimization of the process.
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