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This technique builds on other techniques in this section and shows you how the stego creation process works. You create a new technique, you use it, you find weaknesses in it, and then you figure out ways to fix those weaknesses to build a stronger technique.
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American clients (Murase, 1977; S. Sue et al., 1994). Furthermore, researchers and clinicians are more sensitive to the notion that tremendous diversity in perspectives, needs, and issues exists within each ethnic or minority group (Bernal & Castro, 1994; Fowers & Richardson, 1996; Landrine, 1992; S. Sue et al., 1994). Therefore, generalizing assessment, treatment, or research strategies to all ethnic or minority groups is inappropriate. Acculturation and assimilation factors as well as unique individual differences must be taken into consideration when working with members of diverse groups (APA, 2003b; Lopez et al., 1989; Roysircar & Singh, 2003). More minority representation is needed within the field of psychology to provide better service to minority group members (APA, 2003b; Bernal & Castro, 1994; C. Hall, 1997; Landrine, 1992; Lopez et al., 1989). Although the United States has become a highly diverse nation, the vast majority of clinical psychologists are Caucasian. For example, fewer than 5% of the clinical psychology division of the APA (Division 12) is Asian, Hispanic, or African American. Of all the psychology doctorates awarded in 2001, fewer than 10% were awarded to ethnic minority group members (APA, 2003b). The APA has thus encouraged graduate training programs to increase minority group representation in graduate and postgraduate (e.g., internship and postdoctoral fellowship) admissions. As a result of these efforts, about 20% of incoming doctoral students in psychology are now ethnic minority group members (APA, 2000b; Norcross, Sayette, et al., 2002).
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Fries, J. F. (1990). Reevaluating the therapeutic approach to rheumatoid arthritis: The sawtooth strategy. Journal of Rheumatology, 17(22), 12 15. Fyrand, L., Wichstrom, L., Moum, T., Glennas, A., & Kvien, T. K. (1997). The impact of personality and social support on mental health for female patients with rheumatoid arthritis. Social Indicators Research, 40(3), 285 298. Geenan, R., Godaert, G. L. R., Heijnen, C. J., Vianen, M. E., Wenting, M. L. G., Nederoff, M. G. J., et al. (1998). Experimentally induced stress in rheumatoid arthritis of recent onset: Effects on peripheral blood lymphocytes. Clinical and Experimental Rheumatology, 16, 553 559. Geenan, R., Godaert, G. L. R., Jacobs, J. W. G., Peters, M. L., & Bijlsma, J. W. J. (1996). Diminished autonomic nervous system responsiveness in rheumatoid arthritis of recent onset. Journal of Rheumatology, 23(2), 258 264. Giovengo, S. L., Russell, I. J., & Larson, A. A. (1999). Increased concentrations of nerve growth factor in cerebrospinal uid of patients with bromyalgia. Journal of Rheumatology, 26(7), 1564 1569. Glass, D. C., & Singer, J. E. (1972). Urban stress. New York: Academic Press. Glick, E. N. (1967). Asymmetrical rheumatoid arthritis after poliomyelitis. British Medical Journal, 3, 26 28. Gowans, S. E., deHueck, A., Voss, S., & Richardson, M. (1999). A randomized, controlled trial of exercise and education for individuals with bromyalgia. Arthritis Care and Research, 12(2), 120 128. Griep, E. N., Boersma, J. W., & deKloet, E. R. (1993). Altered reactivity of the hypothalamic-pituitary-adrenal axis in the primary bromyalgia syndrome. Journal of Rheumatology, 20, 469 474. Grossman, C. J., Roselle, G. A., & Mendenhall, C. L. (1991). Sex steroid regulation of autoimmunity. Journal of Steroid Chemistry and Molecular Biology, 40, 649 659. Harrington, L., Af eck, G., Urrows, S., Tennen, H., Higgins, P., Zautra, A., et al. (1993). Temporal covariation of soluble interleukin-2 receptor levels, daily stress, and disease activity in rheumatoid arthritis. Arthritis and Rheumatism, 36(2), 199 203. Harris, E. D. (1993). Clinical features of rheumatoid arthritis. In W. N. Kelley, E. D. Harris, Jr., S. Ruddy, & C. B. Sledge (Eds.), Textbook of rheumatology (4th ed., pp. 874 91). Philadelphia: 1 Saunders. Hazes, J. M. W. (1991). Pregnancy and its effect on the risk of developing rheumatoid arthritis. Annals of Rheumatic Disease, 50, 71 72. Hudson, J. I., Hudson, M. S., Pliner, L. F., & Goldenberg, D. L. (1985). Fibromyalgia and major affective disorder: A controlled phenomenology and family history study. American Journal of Psychiatry, 142(4), 441 446. Hudson, J. I., & Pope, H. G., Jr. (1989). Is bromyalgia a form of affective spectrum disorder Journal of Rheumatology, 19, 15 22.
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Reagents and Materials Sterile Krebs-Ringer Buffer (KRB) (See Section 2.1) KRB with 1 mM EDTA (See Section 2.2) Collagenase, 0.055% (w/v) (See Section 2.3) Autoclaved surgical instruments: partial curve microdissecting forceps, rat tooth tweezers, scalpel, ne sharp-tip scissors, and Mayo blunt scissors Cotton-tipped applicators Lengths of 3-0 silk suture, 6 in., 2 Petri dishes Nonsterile Rat, Female Lewis, 5 8 weeks old, 1 Perfusion system: Water bath to warm perfusate to 39 C out ow temperature and 95% O2 -5% CO2 cylinder to equilibrate perfusate through semipermeable tubing. The end point is attached to a sterile 18-gauge Angiocath catheter Veterinary-grade iso urane anesthesia device (Model 100F) Betadine Ethanol, 70% Beaker, 1 L with lid Hair clippers Vacuum Surgical tray (perforated) with collection tub Protocol (a) Flush perfusion reservoir and tubing with 70% ethanol, then 500 ml UPW, and then 100 ml KRB-EDTA. Add 400 ml KRB-EDTA, clear bubbles from line, and close the perfusion circuit. Set ow rate to 20 ml/min. Allow 15 min for warming and equilibration of KRB-EDTA. (b) Anesthetize rat with vaporizer: Adjust ow rate on the anesthesia device to 1.0 L/min 95% O2 -5% CO2 , and set the iso urane level to 5% v/v. Put exit tubing from the device with nose-cone end in a large beaker and cover, allowing a few minutes for the concentration of iso urane to build. Transfer rat into the beaker, cover, and monitor until fully sedated. (c) After initial sedation, remove rat from beaker and position on paper towels with nose-cone covering its mouth. Maintain portable anesthesia, with iso urane set to 2.5% v/v. Shave abdomen fur and vacuum loose fur. (d) Transfer rat to surgical tray with collection tub underneath, and tape limbs to restrain. Swab abdominal region generously with ethanol, then Betadine.
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