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Fig. 5.8 Effects of 4-nonylphenol on live microsomal metabolism of [4-14C] progesterone. The activity of 6 -hydroxylase (Ohase) is expressed as pmol progesterone metabolite formed per mg microsomal protein per minute in liver microsomes of control, 4-nonylphenol- (NP; single i.p. injection at 1-, 5-, 25- and 125 mg/kg body weight) and oestradiol 17 (E2) treated juvenile salmon. Data are given as mean values SD. *Signi cantly different from control (p < 0.05); **Signi cantly different from control (p < 0.01); Different letters indicate signi cant differences between treatment groups (p < 0.05) (Arukwe et al., 1997b). Reprinted with permission from Environmental Toxicology and Chemistry, 1997. Copyright Society of Environmental Toxicology and Chemistry (SETAC), Pensacola, Florida, USA.

Detector pinhole aperture Laser scan rate and image size Zoom factor Laser selection and laser intensity Gain and offset of the PMT detector Objective lens selection