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First rough estimation of linear range: measure blank + minimum of six independent concentration levels, set up calibration curve and estimate linear range visually Choose CRMs or spiked samples of minimum of six different concentrations within estimated linear range Calculate residual Y values from calibration curve and plot them as funcion of concentration If randomly distributed linearity; if systematic trends non-linearity Calculate relative signals = signal/concentration and plot them as function of concentration If horizontal line linearity; linearity limits correspond to 95% and 105% relative signal values range
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Template files are quite finicky. Here are the rules that you should follow: You must include at least one attribute tag in your template. Each row must be unique don t include the same attribute more than once. You must use only spaces to line up the two columns no tabs! (Lining up the two columns just makes it easier to read.) End each line with a return, including the last line. Don t put any extra spaces after any line, or any extra returns after the last return that is after the last line of text. Each row in the template file becomes a column in the resulting output file. If you choose spacedelimited form for the output file, then spaces do not automatically appear between the columns; as a result, the output files are hard to read. You can place dummy rows in the template file for the purpose of creating spaces in the resulting columns. A typical dummy row looks like this:
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FIGURE 17-15: Copying files.
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Figure 3.29. Resonance energy transfer in uorescence. (a) Resonance energy transfer involves the transfer of light energy from one chromophore to another. The ef ciency of this process is proportional to 1/R 6 , where R is the distance between chromophores. Light from chromophore A (solid arrows) is absorbed by chromophore B. (b) If the emission max of uor A overlaps with the absorbance max of uor B (e.g. at 2 ), this energy can be absorbed ad re-emitted by the latter (dashed arrows in (a)) at 3 allowing R to be calculated from Equation (3.27).
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2.1. Suppose that the Milky Way galaxy is a typical size. containing say 1011 stars, and that galaxies are typically separated by a distance of one megaparsec. Estimate the density of the Universe in SI units. How does this compare with the density of the Earth
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# ls -l /dev total 161 crw-rw---crw------crw-rw---crw-rw---crw------crw------crw------crw------... 1 root video 1 root root 1 root root 1 root root 1 wvh 1 wvh 1 wvh 1 wvh audio audio audio audio 107, 56, 10, 14, 14, 14, 14, 0 2005-03-19 17:01 3dfx 0 2005-03-19 17:01 adb 10 2005-03-19 17:01 adbmouse 14 2005-03-19 17:01 admmidi0 30 2005-03-19 17:01 admmidi1 46 2005-03-19 17:01 admmidi2 62 2005-03-19 17:01 admmidi3 10, 157 2005-03-19 17:01 ac
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